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中华卫生应急电子杂志 ›› 2020, Vol. 06 ›› Issue (01) : 23 -27. doi: 10.3877/cma.j.issn.2095-9133.2020.01.004

所属专题: 文献

论著

丙型肝炎肝硬化患者单核细胞促炎因子与抑炎因子的表达
张永萍1,(), 刘兰1, 张艳君2   
  1. 1. 830000 新疆乌鲁木齐,新疆维吾尔自治区人民医院感染科
    2. 临床医学研究中心
  • 收稿日期:2019-06-24 出版日期:2020-02-18
  • 通信作者: 张永萍
  • 基金资助:
    新疆维吾尔自治区自然科学基金(2015211C207)

Expression of monocytes pro-inflammatory cytokines and anti-inflammatory factors in patients with hepatitisC cirrhosis

Yongping Zhang1,(), Lan Liu1, Yanjun Zhang2   

  1. 1. Department of Infectious Diseases, Xinjiang Autonomous Region People’s Hospital, Urumqi, 830000, China
    2. Clinical Medical Research Center, Xinjiang Autonomous Region People’s Hospital, Urumqi, 830000, China
  • Received:2019-06-24 Published:2020-02-18
  • Corresponding author: Yongping Zhang
  • About author:
    Corresponding author: Zhang Yongping, Email:
引用本文:

张永萍, 刘兰, 张艳君. 丙型肝炎肝硬化患者单核细胞促炎因子与抑炎因子的表达[J/OL]. 中华卫生应急电子杂志, 2020, 06(01): 23-27.

Yongping Zhang, Lan Liu, Yanjun Zhang. Expression of monocytes pro-inflammatory cytokines and anti-inflammatory factors in patients with hepatitisC cirrhosis[J/OL]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2020, 06(01): 23-27.

目的

了解丙肝肝硬化患者单核细胞促炎因子与抑炎因子的表达。

方法

选取2016年1月至2017年5月因丙肝肝硬化入住新疆维吾尔自治区人民医院感染内科的35例患者为丙肝组,健康体检者18例为对照组。常规检测两组患者生化、血脂、血常规等基线资料,提取外周血单核细胞予以培养并提取DNA,测定TNF-α、IL1-β、IL-12-β、IL-10、TGF-β和IL-4 mRNA转录水平及病例组血清HCV-RNA载量。

结果

丙肝组中ALT、AST和TG水平高于对照组,差异有统计学意义(P<0.05);丙肝组中ALB、WBC和PLT低于对照组,差异有统计学意义(P<0.05)。两组患者的TNF-α、IL1-β、IL-12β和IL-4水平无统计学意义(P>0.05),IL-10和TGF-β的表达有统计学意义(P<0.05)。HCV-RNA载量与TNF-α、IL-12β呈正相关(r=0.452,0.442;P<0.05),与IL-10和TGF-β呈负相关(r=-0.431,-0.437,P<0.05)。丙肝组TNF-α与IL1-β、IL-12-β表达呈正相关(r=0.531,0.766,0.448,P<0.05),TNF-α与IL-10、TGF-β呈负相关(r=-0.732,-0.749,P<0.05);IL1-β与IL-10、TGF-β呈负相关(r=-0.438,-0.448,P<0.05);IL-12-β与IL-10、TGF-β呈负相关(r=-0.996,-0.999,P<0.05)。IL-10和TGF-β的表达呈正相关(r=0.999,P<0.05)。

结论

丙肝肝硬化患者单核细胞高表达IL-10和TGF-β。

Objective

To study the expression of monocytes pro-inflammatory cytokines and anti-inflammatory factors in patients with hepatitis C cirrhosis.

Methods

A total of 35 patients with hepatitis C virus-associated cirrhosis were collected from January 2016 to May 2017 in infection department of our hospital. 18 health subjects were enrolled in as control group. Biochemical index, blood lipids, blood routine and other baseline data were detected.

Results

The level of ALT, AST and triglyceride were higher than those of control group, the differences were statistically significant (P<0.05); Albumin, white blood cells and platelet count were lower than those of control group, the differences were statistically significant (P<0.05). The expression of TNF-α, IL1-β, IL-12β and IL-4 was similar in patients group and control group (P>0.05). However, IL-10 and TGF-β expressed in both groups were statistically significant (P<0.05). The HCV-RNA load in patient group was positively correlated with TNF-α, IL-12β (r= 0.452, 0.442, respectively, P<0.05) and negatively correlated with IL-10 and TGF-β (r=-0.732, -0.749, P>0.05). IL-1β and IL-4 displayed no relation with HCV-RNA load. TNF-α displayed a positive correlation with IL1-β and IL-12-β in patient group(r=0.531, 0.7660, 0.448, respectively, P<0.05), TNF-α negatively correlated with IL-10 and TGF-β (r=-0.732, -0.749, respectively, P<0.05). IL-12β also negatively correlated with IL-10 and TGF-β (r=-0.996, -0.999, P<0.05). IL-10 showed a positive correlation with TGF-β cytokines (r=0.999, P<0.05).

Conclusions

IL-10 and TGF-β are highly expressed in monocytes of patients with hepatitis C cirrhosis.

表1 两组基本临床资料比较(±s)
表2 两组细胞因子表达比较(±s)
表3 HCV-RNA载量与细胞因子的偏相关分析
表4 丙肝组中细胞因子间相关分析
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