目的

研究白藜芦醇（RES）对⁶⁰ Coγ射线致小鼠生殖细胞损伤的防护作用。

方法

60 只雄性ICR小鼠随机分成5 组，即正常对照组、照射对照组以及50 mg/kg照射组、100 mg/kg照射组和RES 200 mg/kg照射组，照射前3 d 灌服给药，每天1 次。 除正常对照组外，所有小鼠⁶⁰ Coγ射线全身5.5 Gy辐照1 次，照射后6h 取小鼠睾丸，分离睾丸生精细胞，AnnexinV/PI双荧光染色，采用流式细胞术检测照射后6 h 生精细胞的凋亡率（APO%）；照射后29 d 取小鼠附睾，分离精子，采用伊红染色测定小鼠精子的畸形率（MFM%）。

结果

照射后6 h，与正常对照组比较，照射对照组小鼠睾丸生精细胞的晚期凋亡率显著增高（APO%=31.31 ±6.05，P＜0.05）；与照射对照组比较，RES 各剂量组小鼠睾丸生精细胞的晚期凋亡率显著降低（低剂量APO%=24.07 ±4.18，P＜0.05；中剂量APO%=21.67 ±5.42，P＜0.05；高剂量APO%=18.66 ±5.44，P＜0.05）；照射后21 d，与正常对照组比较，照射对照组小鼠精子畸形率显著增高（MFM%=34.58 ±4.06，P＜0.05），与照射对照组比较，RES 各给药组小鼠精子畸形率显著降低（低剂量MFM%=25.95 ±3.53，P＜0.05； 中剂量MFM%=23.43 ±2.90，P＜0.05； 高剂量MFM% =18.88 ±4.67，P＜0.05）。

结论

RES 可以减轻了γ射线照射引起的生精细胞凋亡以及减少照射引起的小鼠精子畸形。

Objective

To study the protective effects of resveratrol on the ⁶⁰ Co-gamma rays-induced reproductive cell apoptosis in mice.

Methods

A total of 60 male ICR mice were randomized into 5 groups，ie，control group，irradiated group，resveratrol（RES）50 mg/kg irradiated group，RES 100 mg/kg irradiated group and RES 200 mg/kg irradiated group.RES was given for three days，once per day，prior to irradiation.Except for the control group，all mice received systemic irradiation by 60 Co gamma ray with a single dose of 5.5 Gy.The testis was isolated 6 h after irradiation for observing the apoptotic rate of testicular cells by using AnnexinV/PI double fluorescence staining and flow cytometry.The deformity of sperm was detected 29 d after irradiation by using hematoxylin-eosin staining under light microscope.

Results

Compared with the control group，the late apoptosis rate of spermatogenic cells of sperms was 1.31 ±6.05，more significantly increased 6 h after irradiation in irradiation group （P＜0.05）.Compared with the irradiation group，the late apoptosis rate of the spermatogenic cells was significantly lower in other resveratrol treated groups（24.07 ±4.18 for low dose，21.67 ±5.42 for medium dose and 18.66 ±5.44 for high dose，all P＜0.05）.At day 21 after irradiation，the deformity percentage of sperm was 34.58 ±4.06 in the irradiated group，higher than that in the control group （P＜0.05）； the apoptosis rate of spermatogenic cells and deformity percentage of sperms were significantly decreased in the other resveratrol treated groups（25.95 ±3.53 for low dose，23.43 ±2.90 for medium dose and 18.88 ±4.67 for high dose，all P＜0.05）in comparison with the irradiation group.

Conclusion

Resveratrol can effectively alleviate the ⁶⁰ Co-gamma rays-induced apoptosis rate of spermatogenic cells and reduce irradiation-induced deformity percentage of sperms in mice.