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中华卫生应急电子杂志 ›› 2015, Vol. 01 ›› Issue (04) : 20 -23. doi: 10.3877/cma.j.issn.2095-9133.2015.04.007

所属专题: 文献

论著

多器官功能障碍综合征患者血中miRNA表达谱的变化
徐文秀1, 张谦1,()   
  1. 1. 215000 苏州,苏州市中医医院重症医学科
  • 收稿日期:2015-06-17 出版日期:2015-08-18
  • 通信作者: 张谦
  • 基金资助:
    苏州市科技管理局科技发展计划(SZD09138)

Expression profile of miRNAs in blood of patients with multiple organ dysfunction syndrome and their implication

Wenxiu Xu1, Qian Zhang1,()   

  1. 1. Intensive Care Unit, Suzhou Hospital of Traditional Chinese Medicine, Suzhou 215000, China
  • Received:2015-06-17 Published:2015-08-18
  • Corresponding author: Qian Zhang
  • About author:
    Corresponding author: Zhang Qian Email:
引用本文:

徐文秀, 张谦. 多器官功能障碍综合征患者血中miRNA表达谱的变化[J]. 中华卫生应急电子杂志, 2015, 01(04): 20-23.

Wenxiu Xu, Qian Zhang. Expression profile of miRNAs in blood of patients with multiple organ dysfunction syndrome and their implication[J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2015, 01(04): 20-23.

目的

初步了解多器官功能障碍综合征(MODS)患者中全血中miRNA表达谱变化及其意义。

方法

通过对3例肺部感染引起MODS患者及3例对照组患者全血样本进行高通量测序,共监测了1921个已知的miRNA。通过聚类分析从中分离出MODS患者表达异常的miRNA。

结果

所有测序的miRNA中,MODS患者组miR-148a,miR-223表达升高,miR-1273g表达降低。

结论

miR-148a和miR-223增加、miR-1273g的减少参与了MODS的发病过程,将有望成为诊治MODS的新型生物标记物。

Objective

To investigate the expression profil changes of miRNAs in blood of patients with multiple organ dysfunction syndrome (MODS) and discuss their significance.

Methods

Blood samples from 3 patients with MODS caused by the lung infection and 3 patients healthy volunteers were used for high-throughput sequencing. A total of 1921 known miRNAs were screened to separate the abnormally expressed miRNAs through the cluster analysis.

Results

The expression of miR-223 and miR-148a was increased but the expression of miR-1273g was decreased.

Conclusion

miR-148a, miR-223 and miR-1273g may play a role in the pathogenesis of MODS, indicating that they are the potential laboratory markers for diagnosis and treatment of MODS.

图1 高通量测序图
图2 miR-1273g的表达
图3 miR-148a的表达
图4 miR-223的表达
1
Dellinger RP, levy MM, Rhodes A, et al.Surviving sepsis campaign: international guidelines for management of severe sepsis and septicshock[J]. Crit care Med, 2013, 41(2): 580-637.
2
Vasilescu C, Rossi S, Shimizu M, et al.MicroRNA fingerprints identify miR-150 as a plasma prognostic marker in patients with sepsis[J]. PLoS One, 2009, 4(10): e7405.
3
Li X, Zhang Y, Zhang H, et al.miRNA-223 promotes gastric cancer invasion and metastasis by targeting tumor suppressor[J]. Mol Cancer Res, 2011, 9(7): 824-833.
4
Wang L, Jia X, Jiang H, et al.MicroRNAs 185, 96, and 223 Repress Selective High-Density Lipoprotein Cholesterol Uptake through Posttranscriptional Inhibition[J]. Mol Cell Biol, 2013, 33(10): 1956-1964.
5
Kiss T. Small nucleolar RNAs: An abundant group of noncoding RNAs with diverse cellular Functions[J]. Cell, 2002, 109(2): 145-148.
6
Wang H, Meng K, Jun W, et al.Serum miR-574-5p: a prognostic predictor of sepsis patients[J]. Shock, 2012, 37(3): 263-267.
7
Chen Y, Song Y, Wang Z, et al.Altered expression of MiR-148a and MiR-152 in gastrointestinal cancers and Its clinical significance[J]. J Gastroint Surg, 2010, 14(7): 1170-1179.
8
Duursma AM, Kedde M, Schrier M, et al.miR-148 targets human DNMT3b protein coding region[J]. RNA, 2008, 14(5): 872-877.
9
Chen Y, Min L, Zhang XZ, et al. Decreased miRNA-148a is associated with lymph node metastasis and poor clinical outcomes and functions as a suppressor of tumor metastasis in non-small cell lung cancer[J]. Oncol Reports, 2013, 30(4): 1832-1840.
10
Laios A, O′Toole S, Flavin R, et al.Potential role of miR-9 and miR-223 in recurrent ovarian cancer[J]. Mol Cancer, 2008, 7: 35.
11
Li X, Zhang Y, Zhang H, et al.MiRNA-223 promotes gastric cancer invasion and metastasis by targeting tumor suppressor EPB41L3[J]. Mol Cancer Res, 2011, 9(7): 824-833.
12
Xu J, Yao Q, Hou Y, et al.MiR-223/Ect2/p21 signaling regulates osteosarcoma cell cycle progression and proliferation[J]. Biomed Pharmacother, 2013, 67(5): 381-386.
13
Wang J, Yu M, Yu G, et al.Serum miR-146a and miR-223 as potential new biomarkers for sepsis[J]. Biochem Biophys Res Commun, 2010, 394(1): 184-188.
14
Na kanishi K, Nakasa T, Tanaka N, et al.Responses of microRNAs 124a and 223 following spinal cord injury in mice[J]. Spinal Cord, 2010, 48(3): 192-196.
15
Ivashchenko A, Berillo O, Pyrkova A, et al.Binding Sites of miR-1273 Family on the mRNA of Target Genes[J]. Biomed Res In, 2014, 8: 1-14.
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