Abstract:
Objective
To establish an animal model of coagulopathy，and to observe the effect of new therapy of 20AA compound amino acid injection plus high-dose vitamin B6 on coagulation function in the animal model.
Methods
The male SD rats were randomly divided into shame group，saline group and new therapy group，eight each group.The multiple trauma model of rat was established by using Feeney's free fall epidural impact method，plus bilateral femoral fractures and bleeding.The new therapy group was infused with 20AA compound amino acid injection plus high dose of vitamin B6 by jugular vein intubation.The control group was infused with same volume of saline injection.The changes of coagulation function in rats were investigated after 24 hours.
Results
The R value of thrombelastography in the new therapy group［（2.24±0.68）min］was significantly lower than that of the control group［（3.21±0.30）min）］，（t＝-0.98，P＜0.05）.The Angle value of thrombelastography in the new therapy group［（81.98 ±1.78）deg］was significantly higher than that of the control group［（79.54±2.13）deg］，（t＝2.43，P＜0.05）.In traditional coagulation test results，the prothrombin time（PT），activated partial clotting enzyme live time（APTT），thrombin time（TT）in the new therapy group were lower than the values in the control group，but fibrinogen（FIB）was higher than the one of the control group，but the differences were notstatically significant.
Conclusion
The treatment of 20AA compound amino acid injection plus high dose of vitamin B6 can significantly improve the coagulation function in the rat model of multiple trauma.
Key words:
Wounds and juries,
Thrombin,
Amino acids,
Rats,Sprague-dawley
Maoxing Yue, Huaping Liang, Ying Chu, Qing Bao, Xuwen Zheng, Zheng Liu, Wenming Shen, Peigeng Zhou, Ying Li, Xiaoxing Bian, Qingmei Huang. Effects of new therapy of 20 AA compound amino acid plus high dose of vitamin B6 on trauma-induced coagulopathy[J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2015, 01(02): 11-14.