Evaluation on the effect of therapeutic measures to acute lung injury induced by smoke inhalation in airtight cabins

doi:10.3877/cma.j.issn.2095-9133.2016.06.004

Abstract

Abstract:

Objective

To screen and evaluate the therapeutic effects of various treatment on acute lung injury induced by smoke inhalation in airtight cabins.

Methods

78 male SD rats were randomly divided into control group (group A), injury group (group B) and eleven treatment groups (group C~M) with 6 rats in each group. Group B~M were exposed in smoke for 10 min, and C~M groups respectively treated with 30%, 60% and 100% normal pressure oxygen inhalation, heparin sodium atomization, injection of dexamethasone(Dex), vitamine C or N acetyl cysteine (NAC). Blood gas, Carboxyhemoglobin(COHb) and COHb clearance rate (CCR), lung pathological changes, lung wet-to-dry weight ratio (W/D), pulmonary permeability index (PPI), glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) activity and the content of malondialdehyde(MDA) and nitric oxide(NO) were measured at 24h post-exposure to evaluate the effects of various therapeutic measures.

Results

Compared with the group B, the PaO₂ in the group D, E, G and H were increased significantly [ (117.0±1.0) mmHg, (119.2±3.1) mmHg, (115.0±5.7) mmHg, (101.8±3.3) mmHg vs (89.1±1.5) mmHg, P<0.05, respectively]. Compared with the group B, the CCR in the group C, D and E were increased significantly [ (23.1±4.2)%, (24.0±4.9)%, (26.3±4.5)% vs (19.3±2.2)%, P<0.05, respectively]; lung pathological score in the group G, H, L and M was decreased significantly compared with that in the group B [ (2.9±1.0), (5.0±0.4), (4.4±1.5), (5.9±1.4) vs (9.4±1.2), P<0.05, respectively]; lung wet-to-dry weight ratio in the group C, G, H and I were decreased significantly compared with that in the group B [(4.63±0.10), (4.62±0.06), (4.65±0.05), (4.57±0.12) vs (4.88±0.21), P<0.05, respectively]; PPI in the group H was significantly lower than that in the group B [ (2.42±0.38) vs (7.02±1.05), P<0.01]. Compared with the group B, the activity of GSH-Px and SOD significantly increased (P<0.05) and the content of MDA and NO significantly decreased (P<0.05) in the group J, L and M.

Conclusion

100% normal pressure oxygen inhalation, high dose of heparin sodium nebulization, dexamethasone or NAC pretreatment may have a better therapeutic effect on the acute lung injury induced by smoke inhalation in airtight cabins.

Key words: airtight cabin, smoke inhalation, inhalation lung injury

Lei Sun, Sheng Chen, Ying Cai, Hui An, Lin Ao, Huan Yang, Chengyu Tao, Jinyi Liu, Jia Cao. Evaluation on the effect of therapeutic measures to acute lung injury induced by smoke inhalation in airtight cabins[J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2016, 02(06): 350-357.

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Figures/Tables 7

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组别	BALF(×10^－3μg/μL)	血清(μg/μL)	PPI	¹P值	²P值	³P值
A	0.05±0.01	49.1±4.1	1.00±0.27	<0.01	>0.05	<0.01
B	0.25±0.06	35.9±9.8	7.02±1.05	/	/	/
C	0.21±0.01	48.9±9.4	4.42±1.11	>0.05	>0.05	>0.05
D	0.19±0.08	44.3±6.2	4.47±2.49	>0.05	>0.05	>0.05
E	0.23±0.10	49.9±2.4	4.77±2.15	>0.05	>0.05	>0.05
F	0.29±0.04	49.4±3.5	5.85±1.2	>0.05	>0.05	>0.05
G	0.24±0.05	48.5±6.4	5.15±1.62	>0.05	>0.05	>0.05
H	0.12±0.01	51.0±11.6	2.42±0.38	<0.05	>0.05	<0.01
I	0.24±0.07	56.6±9.6	4.33±1.54	>0.05	>0.05	>0.05
J	0.20±0.05	38.8±5.2	5.1±0.84	>0.05	>0.05	>0.05
K	0.25±0.07	46.6±6.9	5.32±0.85	>0.05	>0.05	>0.05
L	0.25±0.07	51.5±2.8	4.9±1.55	>0.05	>0.05	>0.05
M	0.20±0.08	46.5±3.9	4.38±1.86	>0.05	>0.05	>0.05

组别	BALF(×10^－3μg/μL)	血清(μg/μL)	PPI	¹P值	²P值	³P值
A	0.05±0.01	49.1±4.1	1.00±0.27	<0.01	>0.05	<0.01
B	0.25±0.06	35.9±9.8	7.02±1.05	/	/	/
C	0.21±0.01	48.9±9.4	4.42±1.11	>0.05	>0.05	>0.05
D	0.19±0.08	44.3±6.2	4.47±2.49	>0.05	>0.05	>0.05
E	0.23±0.10	49.9±2.4	4.77±2.15	>0.05	>0.05	>0.05
F	0.29±0.04	49.4±3.5	5.85±1.2	>0.05	>0.05	>0.05
G	0.24±0.05	48.5±6.4	5.15±1.62	>0.05	>0.05	>0.05
H	0.12±0.01	51.0±11.6	2.42±0.38	<0.05	>0.05	<0.01
I	0.24±0.07	56.6±9.6	4.33±1.54	>0.05	>0.05	>0.05
J	0.20±0.05	38.8±5.2	5.1±0.84	>0.05	>0.05	>0.05
K	0.25±0.07	46.6±6.9	5.32±0.85	>0.05	>0.05	>0.05
L	0.25±0.07	51.5±2.8	4.9±1.55	>0.05	>0.05	>0.05
M	0.20±0.08	46.5±3.9	4.38±1.86	>0.05	>0.05	>0.05

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