Effect of TLR4 inhibitor on the inflammatory cytokines in microglia induced by LPS

doi:10.3877/cma.j.issn.2095-9133.2018.01.008

Abstract

Abstract:

Objective

To explore the effect of TAK-242, TLR4 inhibitor, on the secreting inflammatory cytokines in BV2 microglia caused by LPS.

Methods

The inflammatory reaction cells were inducedby LPS. (1)Microglias BV2 were cultured with different concentrations of TAK-242, and then , the cell survival rate was detected by cck-8 kit to select the suitable concentration; (2)Microglias BV2 were cultured with different concentrations of lipopolysaccharide(LPS)(0, 0.1, 0.5, 1μg/mL), and then, the TLR4 mRNA expression was detected by Quantitative real-time PCR(qRT-PCR); (3)Microglias BV2 were divided into four groups: Control group, TAK-242 group, LPS group and LPS+ TAK-242 pretreatment group, and then, the TLR4, MYD88, IL-1β, IL-6 mRNA expression were detected by Quantitative real-time PCR(qRT-PCR).

Results

(1)CCK-8: Compared with control group, low concentration of TAK-242 have no effect on the viability of BV2 cells. Compared with LPS group, pretreating of TAK-242(1μM) especially increased the viability of BV2 cells (P<0.05). (2)qRT-PCR: compared with control group, LPS can increase the TLR4 mRNA expression (P<0.05); (3)qRT-PCR: compared with control group, LPS can increase the TLR4mRNA, MyD88mRNA, IL-1βmRNA and IL-6 mRNA expression (P<0.05); Compared with LPS group, pretreating of TAK-242(1μM) can significantly decrease the TLR4mRNA, MyD88mRNA, IL-1βmRNA and IL-6 mRNA expression (P<0.05).

Conclusion

TLR4 signal pathway can be activateed by LPS; TAK-242 can decrease TLR4mRNA, MyD88mRNA, IL-1βmRNA and IL-6 mRNA expression and increase the cells viability.

Key words: TLR4, TAK-242, LPS, BV2 microglia cells

Chao Chen, Lianhong Yang. Effect of TLR4 inhibitor on the inflammatory cytokines in microglia induced by LPS[J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2018, 04(01): 45-49.

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Figures/Tables 7

References 21

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