To study the effect of Da-Huang-Fu-Zi-Tang on the changes of intestinal microfold cells (M cells) in mice with severe acute pancreatitis (SAP), so as to provide a theoretical basis for the clinical prevention of SAP.

Forty healthy SPF C57BL/6J male mice were selected and randomly divided into 4 groups (n=10): blank group, Da-Huang-Fu-Zi-Tang control group, SAP group, Da-Huang-Fu-Zi-Tang treatment group; the experimental group were injected with 20% PH 7.0 L-arginine into the abdominal cavity at a dose of 3.5 g/kg, and the control group were injected with the same amount of normal saline into the abdominal cavity, blank control Group and SAP group were given saline 0.2 mL by enema at 12, 24 and 36 hours after modeling, and the Da-Huang-Fu-Zi-Tang control group and treatment group were given Da-Huang-Fu-Zi-Tang 0.2 mL by enema correspondingly. At 48 hours after modeling, mice were executed and selected. Serum amylase, endotoxin, IL-1t, and TNF-nlasecuted and selected.er modelingnd treatment group were given Da-Huang-Fu-Zi-Tang, and the pathology of the pancreas was analyzed using the modified Schmidt scoring, Chiu’s intestinal mucosal injury scoring criteria were used to histologically score the ileum, and the ileum was taken for immunohistochemical staining to detect GP2 changes, which is a specific protein of M cells.

The blank control group and the Da-Huang-Fu-Zi-Tang control group were generally in good condition after intraperitoneal injection, with good mental state, normal water intake, free movement of limbs, and unaffected actions. After intraperitoneal injection, the mice in the experimental group were generally in poor condition, mentally depressed, unresponsive, sluggish, shortness of breath, abdomen arched back, and reduced drinking water. The levels of amylase, endotoxin, IL-1toxins of amylasewater. l group of Da-Huang-Fu-Zi-Tang were not significantly changed compared with the blank control group (P<0.05). Compared with SAP group, serum amylase, endotoxin, IL-1toxinlaseth SAP groupr. l group of Da-Huang-Fu-Zi-Tang were not significantly changed (P<0.05). HE staining showed that the control group had normal pancreas and ileum. Necrosis of pancreas and ileum tissue was severe in SAP group, and a large number of white blood cell infiltration was seen. In the treatment group, there was mild necrosis of pancreas and ileum tissue, and a small amount of neutrophils and other white blood cells infiltrated. Immunohistochemically, compared with the blank control group, there was no significant change in the expression level of GP2 in the control group of Da-Huang-Fu-Zi-Tang; the expression of GP2 in the SAP group was lower than that of the control group (P<0.05)and the expression level of GP2 in the Tang treatment group increased (P<0.05).

Da-Huang-Fu-Zi-Tang can improve the number and function of M cells as the beginning of intestinal immune response, enhance intestinal immune response, reduce the damage of intestinal immune barrier, and decrease the entry of endotoxin into the blood and so as to improve SAP.