Establishment and evaluation of traumatic coagulation dysfunction model in New Zealand rabbits

doi:10.3877/cma.j.issn.2095-9133.2021.01.002

Abstract

Abstract:

Objective

To establish a New Zealand rabbit model of traumatic coagulation dysfunction, to explore the pathological changes of coagulation dysfunction caused by traumatic hemorrhagic shock and to make a reasonable evaluation of the model to provide a reliable animal experiment method for later research on different types of fluid resuscitation strategies.

Methods

Thirty New Zealand rabbits were used as experimental subjects to establish animal experimental models after successful anesthesia. Blood was drawn 15 minutes before modeling, 15 minutes, 30 minutes and 1 hour after modeling to detect and record blood routine, arterial blood gas indicators, coagulation function, coagulation factors, body temperature, heart rate and other indicators.

Results

Finally, 30 New Zealand rabbits were included in the experiment, and the 1-hour survival rate was 100%. The pH value, partial pressure of oxygen (PO2), and residual alkali (BE) values of the experimental animals began to decrease at 30 min after shock, and a significant decrease occurred at 1 h. Heart rate and K+ concentration increased at 30 min after modeling, and significantly increased at 1 h. There was a significant difference before modeling (P<0.05); body temperature kept no changes. The red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), platelet count (PLT), and white blood cell count (WBC) showed no significant difference within 30 minutes after modeling. Red blood cell count (RBC), 1h after modeling Hemoglobin (HGB), hematocrit (HCT), platelet count (PLT), white blood cell count (WBC) were significantly different from those before modeling (P<0.05). Partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), and fibrinogen (FIB) all decreased after modeling, which were significantly different from that before modeling (P<0.05), and the changes of coagulation factors Ⅱ, Ⅶ, Ⅹ, Ⅸ, and Ⅺ had no significant statistical significance.

Conclusion

This study proves the feasibility of this experimental method to establish an animal model of traumatic coagulopathy, providing a simple, practical and stable model for the study of traumatic coagulopathy, which lays a solid foundation for the later discussion of different types of fluid resuscitation strategies.

Key words: Wounds and injuries, Coagulation dysfunction, Animal model

Yang Wu, Shijian Yi, Lanlan Li, Qiankun Liang, Songyan Wu, yue Xiao. Establishment and evaluation of traumatic coagulation dysfunction model in New Zealand rabbits[J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2021, 07(01): 8-12.

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References 22

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时间	心率(次/分)	体温(℃)	PH值	PO₂(mmHg)	K^＋浓度(mmol/L)	BE(mmol/L)
建模前15 min	185±4.2	38.4±0.3	7.43±0.07	98.9±8.14	4.51±0.36	-6.83±4.97
建模后15 min	189±5.1	38.1±0.8	7.39±0.04	98.1±7.42	4.75±0.12	-6.98±4.28
建模后30 min	211±7.3	37.8±0.6	7.37±0.09	93.2±6.88	5.22±0.81	-9.25±5.22
建模后1 h	209±5.9	37.4±0.6	7.36±0.09	90.2±6.94	5.31±0.17	-11.21±3.88
F值	24.35	1.21	3.78	10.52	6.34	32.51
P值	<0.05	>0.05	<0.05	<0.05	<0.05	<0.05

时间	心率(次/分)	体温(℃)	PH值	PO₂(mmHg)	K^＋浓度(mmol/L)	BE(mmol/L)
建模前15 min	185±4.2	38.4±0.3	7.43±0.07	98.9±8.14	4.51±0.36	-6.83±4.97
建模后15 min	189±5.1	38.1±0.8	7.39±0.04	98.1±7.42	4.75±0.12	-6.98±4.28
建模后30 min	211±7.3	37.8±0.6	7.37±0.09	93.2±6.88	5.22±0.81	-9.25±5.22
建模后1 h	209±5.9	37.4±0.6	7.36±0.09	90.2±6.94	5.31±0.17	-11.21±3.88
F值	24.35	1.21	3.78	10.52	6.34	32.51
P值	<0.05	>0.05	<0.05	<0.05	<0.05	<0.05

时间	RBC(×10¹²/L)	WBC(×10⁹/L)	PLT(×10⁹/L)	HGB(g/L)	HCT(%)
建模前15 min	5.48±0.47	9.52±1.91	363.75±80.13	113.41±10.56	35.86±1.27
建模后15 min	5.18±0.71	9.22±2.94	323.91±65.11	108.11±13.27	29.96±2.01
建模后30 min	4.34±0.25	10.12±3.11	271.89±74.55	86.23±11.26	28.21±1.55
建模后1 h	3.18±0.19	11.48±2.45	221.88±60.51	64.91±10.22	38.24±2.21
F值	22.34	1.65	8.76	32.22	23.65
P值	<0.05	>0.05	<0.05	<0.05	<0.05

时间	RBC(×10¹²/L)	WBC(×10⁹/L)	PLT(×10⁹/L)	HGB(g/L)	HCT(%)
建模前15 min	5.48±0.47	9.52±1.91	363.75±80.13	113.41±10.56	35.86±1.27
建模后15 min	5.18±0.71	9.22±2.94	323.91±65.11	108.11±13.27	29.96±2.01
建模后30 min	4.34±0.25	10.12±3.11	271.89±74.55	86.23±11.26	28.21±1.55
建模后1 h	3.18±0.19	11.48±2.45	221.88±60.51	64.91±10.22	38.24±2.21
F值	22.34	1.65	8.76	32.22	23.65
P值	<0.05	>0.05	<0.05	<0.05	<0.05

时间	PT(s)	TT(s)	APTT(s)	FIB (g/L)	Ⅱ因子(%)	Ⅶ因子(%)	Ⅹ因子(%)	Ⅸ因子(%)	Ⅺ因子(%)
建模前15 min	10.70±1.89	18.4±2.1	18.74±1.05	2.34±0.26	93.74±31.05	67.74±12.46	37.74±11.08	152.14±42.29	57.74±19.68
建模后15 min	11.23±1.54	18.6±1.9	22.12±2.97	2.14±0.44	94.38±21.34	61.33±14.54	32.56±13.53	137.22±52.08	50.30±16.21
建模后30 min	12.31±1.08	22.7±4.2	31.67±4.19	1.95±0.31	89.44±38.78	65.12±11.96	33.21±16.32	143.89±64.21	53.21±17.54
建模后1 h	22.76±1.49	32.1±6.4	38.28±1.95	1.54±0.76	92.51±30.01	69.31`±13.79	36.84±19.48	150.31±32.88	55.72±18.76
F值	10.53	5.45	9.26	8.63	2.08	1.89	1.66	1.53	1.87
P值	<0.05	<0.05	<0.05	<0.05	>0.05	>0.05	>0.05	>0.05	>0.05

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