Abstract:

Objective

To explore the effects of the traditional Chinese medicine，parenteral solution of Xuebijing，on the inflammatory reaction and immune function after traumatic brain injury.

Methods

In the animal experimental study，30 healthy adult male Sprague-Dawley rats were randomly devided into 3 groups：one normal control group and two Xuebijing intervention groups.Physiological saline and Xuebijing were injected separately every 12 hours until 7 d after trauma.Serum samples were collected at 6 h，24 h，3 d，5 d，7 d after trauma，and the S100B protein and IL-6 content was detected with ELISA.In clinical trials，sixty patients（GCS≤8 points）were randomly devided into two groups of A（treated with conventional therapy and Xuebijing）and B（treated with conventional therapy alone）.Patients of group A were injected with Xuebijing every 8 hours until 7 d after trauma.Serum samples were collected at 1 d，4 d，and 8 d after trauma，and then cellular immunity and humoral immune function were detected.

Results

In the animal experiments，the serum S100B protein（26.25±16.35）pg/ml、（38.31±8.89）pg/ml、（35.61±18.40）pg/ml、（28.91±13.65）pg/ml、（26.82±10.58）pg/ml and IL-6 content（46.77±14.30）pg/ml、（55.96±37.81）pg/ml、（59.14±36.60）pg/ml、（55.22±22.22）pg/ml、（46.73±13.52）pg/ml significantly increased after traumatic brain injury（t＝8.792，P＜0.001）.And compared with the control group，it was obviously inhibition in treatment group with using Xuebijing early after traumatic brain injury（t＝12.336，P＜0.001）.In the clinical trials，the HLA-DR and CD3 ＋of CD4 ＋were 8d respectively（57.10±7.09）%、（55.09±3.15）%、（29.68.±3.16）% significantly higher and the CD8^＋4 d，8 d，respectively（21.33±2.42）%、（23.30±3.35）%significantly lowerthan the control group（t＝3.135，P＜0.05）.

Conclusions

Xuebijing used in the early stage of traumatic brain injury might have a neuroprotective effect and improve immune function.Accordingly，it could improve the prognosis of severe traumatic brain injury.

Key words: raniocerebral tranma, Inflammatory, Immunity