Abstract:

Objective

To explore the diagnosis and treatment compound amino acid and vitamin B₆ nitrogen tetroxide inhalation poisoning the curative effect of pulmonary edema.

Methods

ICR mice 24，weight 23-25 g（n＝8），were randomly divided into three groups：control group，poisoned group，and treatment group.The mice were put in the120 L exposed cabinet to inhalate nitrogen oxides as environmental exposure mode.Bloods were collected by the eye-excised method at 48 h time point，the serum was separated by-70℃preservation，and the ELISA was used to detect the contents of ANPand SP-D.

Results

Serum ANPand SP-D were（221.74±30.82）mg/L and（24.09±2.79）mg/L in control group，（313.46±31.58）mg/Land（20.40±4.27）mg/L in the poisoned group，and（262.03±26.50）mg/L and（23.20±2.84）mg/L in the treatment group，respectively.We used SPSS20.0 software to perform single factor analysis of variance，and P＜0.05 was set as statistical significance.The difference was statistically significant between the poisoned group and the control group in the ANP and SP-D levels（t _ANP ＝7.85，t _SP-D ＝6.89，P＜0.05），and the same to that between the poisoned group and the treatment group in the ANPand SP-D levels（t _ANP ＝4.52，t _SP-D ＝3.67，P＜0.05），but no significant difference existed in the level of TGF-β1.

Conclusions

It leads to impairment of pulmonary edema in short time exposure of nitrogen oxides.To detect the level of ANP and SP-D could be helpful to study the pulmonary edema.Compound amino acid and vitamin B₆ could obviously improve the level of serum ANP and SP-D in the animals poisoned by N₂O₄，and disease outcome，which have a certain reference value in the treatment of such patients.

Key words: Nitrogen oxides, Pulmonary edema, Compound amino acid, Vitamin B₆