Protective effects of VB6 and fenuoan on peripheral blood cells of X-ray irradiated mice and their significance

doi:10.3877/cma.j.issn.2095-9133.2020.01.003

Abstract

Abstract:

Objective

To observe the protective effect of vitamin B₆ (VB₆)and Fengnuoan (20AA compound amino acid) on peripheral blood cells of X-ray irradiated mice.

Methods

The mice were randomly divided into five groups: control group, model group, VB₆ group, Fengnuoan group and combined group. Except the control group, the mice in the other four groups received X-ray irradiation. From the 4th day before irradiation to the 10th day after irradiation, the mice in control group and the model group were injected with normal saline intraperitoneally, the mice in VB₆ group, Fengnuoan group and combined group were injected intraperitoneally with VB₆, fenoan, and the compound solution of VB₆ and fenoan respectively. The mice were killed on the 11 th day after irradiation, and the blood routine and peripheral blood lymphocyte subsets were detected.

Results

Compared with the control group, the total number of leukocytes, neutrophils, lymphocytes, monocytes, erythrocytes, platelets, NK cells, total T cells, CD4⁺ and CD8⁺ T cells and hemoglobin concentration in the peripheral blood of X-ray irradiated mice were significantly reduced (P<0.05), the ratio of CD4 to CD8 was significantly increased. Fengnuoan, VB₆ and compound of Fengnuoan and VB₆ could increase the total number of leukocytes, lymphocytes, platelets, NK cells, total T cells, B cells, CD4⁺ and CD8⁺ T cells of X-ray irradiated mice (P<0.05). Compound was better than VB₆ in increasing the number of total T cell [(41.94%±10.90%) vs (32.14%±5.86%)] and CD4⁺ T cell [(33.11%±10.00%) vs (25.01%±4.64%)], and better than Fengnuoan in increasing the total number of leukocytes [(6.3±0.6)×10⁹/L vs (3.7±0.5)×10⁹/L]and lymphocytes [(5.3±0.4)×10⁹/L vs (3.4±0.3)×10⁹/L].

Conclusion

Fengnuoan and VB₆ could protect the peripheral blood cells of mice irradiated by X-ray. The protective effect of combined VB₆ and fenuoan is better than that of single application.

Key words: Ionizing radiation, Vitamin B₆, Fengnuoan, Hematopoietic function, Lymphocyte subsets

Shaoyan Si, Xiaodie Zhu, Shujun Song, Yingying Wu, Yaya Qin, Maoxing Yue. Protective effects of VB₆ and fenuoan on peripheral blood cells of X-ray irradiated mice and their significance[J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2020, 06(01): 17-22.

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References 24

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组别	白细胞百分数(%)			白细胞计数(×10⁹/L)				血细胞计数(×10¹²/L)		血红蛋白(g/L)
组别	中性粒细胞	淋巴细胞	单核细胞	白细胞总数	中性粒细胞	淋巴细胞	单核细胞	红细胞	血小板	血红蛋白(g/L)
对照组	13.9±8.7	79.9±5.4	5.1±4.9	9.1±1.5	1.3±0.8	7.3±1.4	0.4±0.4	9.1±0.4	1041.4±128.6	158.2±3.1
模型组	7.7±5.5	83.2±14.1	6.1±2.4	2.6±0.6^a	0.2±0.2^a	2.1±0.2^a	0.2±0.2	7.7±0.5^a	577.7±65.4^a	139.0±7.4^a
维生素B₆组	14.1±6.1^c	65.1±6.3^abc	19.1±11.0	6.5±1.1^ab	0.7±0.7	4.2±0.6^ab	1.3±1.1^c	8.0±0.6^a	807.3±122.9^ab	143.2±11.5^a
丰诺安组	5.3±4.8	88.5±2.6^a	4.8±3.2	3.7±0.5^abc	0.2±0.1^ac	3.4±0.3^abc	0.4±0.1	8.0±0.2^a	792.4±52.7^ab	146.9±4.8
联合组	7.0±5.6	86.0±3.7	5.4±4.0	6.3±0.6^ab	0.6±0.3	5.3±0.4^b	0.4±0.3	8.0±0.3^a	736.0±71.0^ab	141.6±6.5^a
F值	3.54	3.80	1.90	18.44	3.18	11.88	2.75	2.77	12.99	2.88
P值	<0.05	<0.05	>0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05

组别	白细胞百分数(%)			白细胞计数(×10⁹/L)				血细胞计数(×10¹²/L)		血红蛋白(g/L)
组别	中性粒细胞	淋巴细胞	单核细胞	白细胞总数	中性粒细胞	淋巴细胞	单核细胞	红细胞	血小板	血红蛋白(g/L)
对照组	13.9±8.7	79.9±5.4	5.1±4.9	9.1±1.5	1.3±0.8	7.3±1.4	0.4±0.4	9.1±0.4	1041.4±128.6	158.2±3.1
模型组	7.7±5.5	83.2±14.1	6.1±2.4	2.6±0.6^a	0.2±0.2^a	2.1±0.2^a	0.2±0.2	7.7±0.5^a	577.7±65.4^a	139.0±7.4^a
维生素B₆组	14.1±6.1^c	65.1±6.3^abc	19.1±11.0	6.5±1.1^ab	0.7±0.7	4.2±0.6^ab	1.3±1.1^c	8.0±0.6^a	807.3±122.9^ab	143.2±11.5^a
丰诺安组	5.3±4.8	88.5±2.6^a	4.8±3.2	3.7±0.5^abc	0.2±0.1^ac	3.4±0.3^abc	0.4±0.1	8.0±0.2^a	792.4±52.7^ab	146.9±4.8
联合组	7.0±5.6	86.0±3.7	5.4±4.0	6.3±0.6^ab	0.6±0.3	5.3±0.4^b	0.4±0.3	8.0±0.3^a	736.0±71.0^ab	141.6±6.5^a
F值	3.54	3.80	1.90	18.44	3.18	11.88	2.75	2.77	12.99	2.88
P值	<0.05	<0.05	>0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05

组别	NK细胞(%)	B细胞(%)	Treg细胞(%)	总T细胞(%)	CD4^＋T细胞(%)	CD8^＋T细胞(%)	CD4/CD8
对照组	4.38±1.17	18.58±1.98	3.11±0.76	76.56±2.29	50.62±4.13	25.97±2.53	1.97±0.32
模型组	6.00±2.47	60.71±8.63^a	6.93±1.72^a	29.91±7.68^a	23.44±6.85^a	6.57±1.40^a	3.59±0.97^a
维生素B₆组	6.51±2.04	59.56±6.50^a	5.82±2.03^a	32.14±5.86^ac	25.01±4.64^ac	7.24±1.79^a	3.55±0.82^a
丰诺安组	9.49±4.42^a	52.39±8.29^a	5.65±0.74^a	34.96±8.96^ac	27.71±8.55^a	7.35±1.33^a	3.83±1.33^a
联合组	10.06±1.41^ab	46.87±11.36^ab	4.95±0.77^ab	41.94±10.90^ab	33.11±10.00^a	8.92±2.00^a	3.81±1.09^a
F值	4.93	22.66	6.25	30.05	11.61	108.69	3.24
P值	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05

组别	NK细胞(%)	B细胞(%)	Treg细胞(%)	总T细胞(%)	CD4^＋T细胞(%)	CD8^＋T细胞(%)	CD4/CD8
对照组	4.38±1.17	18.58±1.98	3.11±0.76	76.56±2.29	50.62±4.13	25.97±2.53	1.97±0.32
模型组	6.00±2.47	60.71±8.63^a	6.93±1.72^a	29.91±7.68^a	23.44±6.85^a	6.57±1.40^a	3.59±0.97^a
维生素B₆组	6.51±2.04	59.56±6.50^a	5.82±2.03^a	32.14±5.86^ac	25.01±4.64^ac	7.24±1.79^a	3.55±0.82^a
丰诺安组	9.49±4.42^a	52.39±8.29^a	5.65±0.74^a	34.96±8.96^ac	27.71±8.55^a	7.35±1.33^a	3.83±1.33^a
联合组	10.06±1.41^ab	46.87±11.36^ab	4.95±0.77^ab	41.94±10.90^ab	33.11±10.00^a	8.92±2.00^a	3.81±1.09^a
F值	4.93	22.66	6.25	30.05	11.61	108.69	3.24
P值	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05

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