To establish an animal model of coagulopathy, and to observe the effect of compound amino acid injection plus high-dose vitamin B6 on coagulation function in an animal model.

The experimental animals were rabbits, which were divided into the control group (10), the coagulation disorder group (30), and the coagulation disorder treatment group (30). The coagulation disorder group and the coagulation disorder treatment group were treated with trauma (laparotomy) and foot artery bloodletting was performed to establish a rabbit trauma blood loss model. In the treatment group, the same amount of normal saline was injected via the ear vein; while in the treatment group, the same amount of compound amino acid injection plus high-dose vitamin B6 was injected via the ear vein. Blood samples were collected at 12 hours, 24 hours, 48 hours and 72 hours after the treatment, and the liver histopathology was observed at 72 hours.

After treatment, compared with the three groups of coagulopathy treatment group, coagulation disorder group, and control group, the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) were lower than those of coagulation in the disorder groups, and the difference was statistically significant; but the difference was not statistically significant from that of the control group; the fibrinogen (FIB) results of the groups within 72 hours after injury were not statistically significant. The coagulation factor Ⅱ, Ⅶ, Ⅸ, and X activities of each group were measured at 12 and 24 hours after injury. The coagulopathy treatment group had higher activity than the coagulation disorder group, and the difference was statistically significant (P <0.05); compared with the control group no statistical significance was detected. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and bilirubin (TBIL) were measured within 72 hours after injury. These in coagulopathy treatment group was lower than those of coagulation disorder group, and the difference was statistically significant (P<0.05); For coagulation there was no statistically significant difference in factor Ⅺ activity and platelet (PLT)within 72 hours after injury. After 72 hours, liver pathological examination showed that the hepatocytes in the coagulation disorder group were severely swollen, spherical, and the cytoplasm was almost completely transparent, showing a balloon-like change; the hepatocytes in the coagulation disorder treatment group were edema and the cytoplasm was reticular; the liver cells in the control group were slight edema.

Compound amino acids combined with high-dose vitamin B6 can significantly improve the liver function and coagulation function of rabbits after traumatic blood loss, increase the activity of coagulation factors and effectively protect liver cells.