Abstract:

Objective

To study the protective effects of resveratrol on the ⁶⁰ Co-gamma rays-induced reproductive cell apoptosis in mice.

Methods

A total of 60 male ICR mice were randomized into 5 groups，ie，control group，irradiated group，resveratrol（RES）50 mg/kg irradiated group，RES 100 mg/kg irradiated group and RES 200 mg/kg irradiated group.RES was given for three days，once per day，prior to irradiation.Except for the control group，all mice received systemic irradiation by 60 Co gamma ray with a single dose of 5.5 Gy.The testis was isolated 6 h after irradiation for observing the apoptotic rate of testicular cells by using AnnexinV/PI double fluorescence staining and flow cytometry.The deformity of sperm was detected 29 d after irradiation by using hematoxylin-eosin staining under light microscope.

Results

Compared with the control group，the late apoptosis rate of spermatogenic cells of sperms was 1.31 ±6.05，more significantly increased 6 h after irradiation in irradiation group （P＜0.05）.Compared with the irradiation group，the late apoptosis rate of the spermatogenic cells was significantly lower in other resveratrol treated groups（24.07 ±4.18 for low dose，21.67 ±5.42 for medium dose and 18.66 ±5.44 for high dose，all P＜0.05）.At day 21 after irradiation，the deformity percentage of sperm was 34.58 ±4.06 in the irradiated group，higher than that in the control group （P＜0.05）； the apoptosis rate of spermatogenic cells and deformity percentage of sperms were significantly decreased in the other resveratrol treated groups（25.95 ±3.53 for low dose，23.43 ±2.90 for medium dose and 18.88 ±4.67 for high dose，all P＜0.05）in comparison with the irradiation group.

Conclusion

Resveratrol can effectively alleviate the ⁶⁰ Co-gamma rays-induced apoptosis rate of spermatogenic cells and reduce irradiation-induced deformity percentage of sperms in mice.

Key words: Resveratrol, Irradiation, Reproductive cell, Apoptosis, Deformity