To investigate the protective effect of Dahuangfuzi decoction on intestinal epithelial tight junction after hemorrhagic shock.

Seventy-two SD rats were allocated to sham operation group, hemorrhagic shock controlgroup, Dahuang Fuzi Decotion group according to random number table, and each group was divided into three subgroups according to the time point of 0, 6, 12 h after HS and resuscitation. The rat model of hemorrhagic shock was induced by using the improved Wiggers method.Model group and Dahuang Fuzi Decotion group were given physiological saline or Dahuang Fuzi Decotion 2ml retention enema after the post-resuscitation. At three time points, the level of intestinal fatty acid binding protein (IFABP) in the serum was tested by ELISA method, and the levels of serum endotoxin (LPS) was detected by dynamic turbidimetric method. The morphological changes of small intestinal mucosa in rats was observed under light microscope, while p-VASP and ZO-1 expression was observed by Western blot and immunohistochemical method.

Compared with model group, the intestinal blood flow was increased [ (119.28±6.50) bpu: (99.99±0.81) bpu, P<0.01], serum endotoxin (LPS), fatty acid binding protein (IFABP) content gradually reduced [(21.23±3.33) ng/mL: (27.09±3.55) ng/mL, P<0.01; (0.420±0.059) ng/mL: (0.645±0.058) ng/mL, P<0.01] were reduced, and the intestinal mucosa damage[(1.78±0.24): (3.64±0.25), P<0.01] was alleviated, p-VASP protein expression [(0.106±0.030): (0.297±0.045), P<0.01], ZO-1 expression [(0.82±0.047) ng/mL: (0.61±0.041) ng/mL, P<0.01] was up-regulated.

Dahuangfuzi decoction can significantly protect the intestinal mucosal barrier function by regulating p-VASP, ZO 1 protein expression after hemorrhagic shock and resuscitation.