Effects of collagen peptides with different administration routes on immune functions of immunosuppressed mice

doi:10.3877/cma.j.issn.2095-9133.2018.04.006

Abstract

Abstract:

Objective

To find out the best administration route of collagen peptides to improve the immune functions of mice.

Methods

Mice were randomly divided into 2 groups: intragastric administration group and intraperitoneal injection group. Then, the two groups were respectively divided into 5 subgroups: control group, model group and 100, 300, 600 mg collagen peptides groups, 6 mice in each group. Immunosuppression were induced in mice of model groups and collagen peptides groups with glucocorticoid. Mice in collagen peptides groups of intragastric administration group and intraperitoneal injection group were respectively intragastrically and intraperitoneally administrated 100, 300, 600 mg collagen peptides per kilogram by for 7 days. The mice were killed on the tenth days. Thymus and spleen indexes, white cells count and classification, the ratios of CD⁴ to CD⁸ and T lymphocyte proliferations of spleens were respectively analyzed.

Results

Compared with control group, the spleen indexes, numbers and percentages of leukocytes and percentages of lymphocytes and ratios of CD⁴⁺ to CD⁸⁺ were improved in mice after intraperitoneally administrated with different dose of collagen peptides from low or middle dose (P<0.05) and were not significantly different with control group (P>0.05). The above parameters except spleen indexes were improved in mice of 600 mg collagen peptides groups after intragastric administration (P<0.05), but they were still significantly different with control group (P<0.05).

Conclusion

Intraperitoneal injection is superior to intragastric administration with collagen peptides in improvements of the immune functions in immunosuppressed mice.

Key words: Collagen peptides, Intraperitoneal injection, Intragastric administration, Immune function

Shaoyan Si, Bingxin Xu, Yingying Wu, Yaya Qin, Kui Wang, Shujun Song. Effects of collagen peptides with different administration routes on immune functions of immunosuppressed mice[J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2018, 04(04): 224-228.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhwsyjdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-9133.2018.04.006

https://zhwsyjdzzz.cma-cmc.com.cn/EN/Y2018/V04/I04/224

Figures/Tables 5

References 16

1	李承威，马淑霞，王春敏，等．胶原蛋白肽增强小鼠免疫力的实验研究[J]．中国微生态学杂志，2013，25(4):387-389.
2	中国研究型医院学会卫生应急学专业委员会/中国中西医结合学会灾害医学专业委员会．人类免疫缺陷病毒感染患者创伤后救治的专家共识[J/CD]．中华卫生应急电子杂志，2017，3(6):321-324.
3	Zhang Y, Kouguchi T, Shimizu M，et al.Chicken collagen hydrolysate protects rats from hypertension and cardiovascular damage[J]．J Med Food，2010，13(2):399-405.
4	König D, Oesser S, Scharla S，et al.Specific collagen peptides improve bone mineral density and bone markers in postmenopausal women - a randomized controlled study[J]．Nutrients，2018，10(1):E97.
5	Ma Q, Liu Q, Yuan L，et al.Protective effects of LSGYGP from fish skin gelatin hydrolysates on UVB-induced MEFs by regulation of oxidative stress and matrix metalloproteinase activity[J]．Nutrients，2018，10(4): E420.
6	Castro GA, Maria DA, Bouhallab S，et al.In vitro impact of a whey protein isolate (WPI) and collagen hydrolysates (CHs) on B16F10 melanoma cells proliferation[J]．J Dermatol Sci，2009，56(1): 51-57.
7	王毅虎，张兵，王富荣，等．胶原蛋合增强免度力功能研究[J]．明胶科学与技术，2015，35(3):137-143.
8	邓超，汤鲁宏，陈伟，等．霞水母胶原蛋白活性肽对小鼠免疫功能的影响[J]．安徽农业科学，2009，37(8):3557-3558,3563.
9	王凤林，万林春，李谨谨，等．暹罗鳄鱼鳞胶原蛋白多肽对小鼠免疫功能的影响[J]．中国医药科学，2011，1(24):34-35.
10	丁进锋，苏秀榕，李妍妍，等．海蜇胶原蛋白肽的免疫活性的研究[J]．水产科学，2011，30(6):359-361.
11	Fan J, Zhuang Y, Li B．Effects of collagen and collagen hydrolysate from jelly fish umbrella on histological and immunity changes of mice photo aging [J]．Nutrients，2013，5(1):223-233.
12	司少艳，刘俊丽，郭燕川，等．腹腔注射胶原蛋白肽增强糖皮质激素免疫抑制小鼠的免疫功能[J]．细胞与分子免疫学杂志，2014，30(6):608-610.
13	Monteiro P, Perez I, Laguno M，et al.Dual therapy with etravirine plus raltegravir for virologically suppressed HIV-infected patients: a pilot study [J]．J Antimicrob Chemother，2014，69(3):742-748.
14	冉立峰，杨炜．不同营养方式对老年食管癌患者术后免疫功能的影响[J]．免疫学杂志，2013，29(8):681-684.
15	Wang WJ, Tao Z, Gu W，et al.Variation of blood T lymphocyte subgroups in patients with non-small cell lung cancer [J]．Asian Pac J Cancer Prev，2013，14(8):4671-4673.
16	陈俊德，易瑞灶，陈晖．鱼胶原蛋白及其活性肽的研究进展[J]．中国海洋药物杂志，2009，28(4):51-56.

动物分组	脾脏指数		胸腺指数
动物分组	灌胃	腹腔注射	灌胃	腹腔注射
对照组	4.2±0.7	4.3±0.8	2.8±0.4	2.9±0.8
模型组	2.9±0.6^a	2.8±0.3^a	1.0±0.2^a	1.0±0.1^a
100 mg/kg组	2.6±0.1^a	3.6±0.6^b	1.3±0.2^a	1.4±0.4^a
300 mg /kg组	2.8±0.6^a	3.8±0.3^b	1.7±0.5^ab	1.5±0.4^ab
600 mg /kg组	2.7±0.3^a	4.5±0.3^b	1.9±0.4^ab	1.8±0.5^ab

动物分组	脾脏指数		胸腺指数
动物分组	灌胃	腹腔注射	灌胃	腹腔注射
对照组	4.2±0.7	4.3±0.8	2.8±0.4	2.9±0.8
模型组	2.9±0.6^a	2.8±0.3^a	1.0±0.2^a	1.0±0.1^a
100 mg/kg组	2.6±0.1^a	3.6±0.6^b	1.3±0.2^a	1.4±0.4^a
300 mg /kg组	2.8±0.6^a	3.8±0.3^b	1.7±0.5^ab	1.5±0.4^ab
600 mg /kg组	2.7±0.3^a	4.5±0.3^b	1.9±0.4^ab	1.8±0.5^ab

动物分组	白细胞总数	中性粒细胞百分比	中性粒细胞绝对值	淋巴细胞百分比	淋巴细胞绝对值	CD^4＋/CD^8＋
对照组	8.6±1.1	9.2±0.2	0.8±0.1	89.9±0.5	7.7±1.0	4.3±0.9
模型组	4.5±1.2^a	41.9±10.9^a	1.8±0.9^a	58.1±10.9^a	2.5±0.8^a	1.8±0.1^a
100 mg/kg组	3.7±0.6^a	20.1±5.7^ab	0.7±0.3^b	77.6±5.2^ab	3.4±0.8^a	2.1±0.4^ab
300 mg/kg组	3.9±0.8^a	12.6±2.3^ab	0.5±0.1^ab	84.5±2.0^ab	3.1±0.7^a	2.9±0.3^ab
600 mg/kg组	4.3±1.3^a	10.8±3.7^b	0.5±0.2^ab	86.7±3.6^b	3.4±0.6^a	3.2±0.6^b

动物分组	白细胞总数	中性粒细胞百分比	中性粒细胞绝对值	淋巴细胞百分比	淋巴细胞绝对值	CD^4＋/CD^8＋
对照组	8.6±1.1	9.2±0.2	0.8±0.1	89.9±0.5	7.7±1.0	4.3±0.9
模型组	4.5±1.2^a	41.9±10.9^a	1.8±0.9^a	58.1±10.9^a	2.5±0.8^a	1.8±0.1^a
100 mg/kg组	3.7±0.6^a	20.1±5.7^ab	0.7±0.3^b	77.6±5.2^ab	3.4±0.8^a	2.1±0.4^ab
300 mg/kg组	3.9±0.8^a	12.6±2.3^ab	0.5±0.1^ab	84.5±2.0^ab	3.1±0.7^a	2.9±0.3^ab
600 mg/kg组	4.3±1.3^a	10.8±3.7^b	0.5±0.2^ab	86.7±3.6^b	3.4±0.6^a	3.2±0.6^b

动物分组	白细胞总数	中性粒细胞百分比	中性粒细胞绝对值	淋巴细胞百分比	淋巴细胞绝对值	CD^4＋/CD^8＋
对照组	8.2±0.7	8.8±0.4	0.8±0.2	90.8±6.0	7.4±0.8	4.5±0.5
模型组	4.9±0.6^a	42.5±0.2^a	2.1±0.5	56.4±4.9^a	2.8±0.4^a	1.5±0.4^a
100 mg/kg组	4.6±0.1^a	43.3±0.2^a	2.1±0.3	55.2±9.0^a	2.5±0.4^a	1.6±0.4^a
300 mg/kg组	4.8±0.6^a	41.7±0.5^a	2.1±0.3	56.9±6.1^a	2.7±0.5^a	1.9±0.3^a
600 mg/kg组	4.7±0.3^a	32.9±0.4^ab	1.6±0.3^ab	65.2±5.4^ab	3.1±0.4^ab	2.3±0.3^ab

Please choose a citation manager

Content to export