Protective effect of cardiopulmonary resuscitation on brain mitochondrial function in a rat model of ventricular fibrillation

doi:10.3877/cma.j.issn.2095-9133.2015.06.006

Abstract

Abstract:

Objective

To investigate the protective effect of cardiopulmonary resuscitation on brain mitochondrial function in a rat model of cardiac arrest.

Methods

Ventricular fibrillation(VF) was electrically induced via alternating current delivered to the right ventricular endocardium. The rats were randomized into three groups, ie, control group, ischemia group and CPR group. Clark oxygen electrode was used to measure mitochondrial respiratory function. Mitochondrial ultrastructure was observed under transmission electron microscope. Hippocampal mitochondria was removed rapidly and quick-frozen in liquid nitrogen for further HPLC analysis.

Results

The levels of State Ⅲ O₂ consumption and RCR in CPR group were significantly less than that in control group. There was no morphological difference in between two groups. The content of PCr and ATP in both ischemia group and CPR group were less than that in control group.

Conclusions

Cardiac arrest causes severe injuries in brain mitochondrial respiratory function. CPR could improve mitochondrial function and alleviate ischemic neurons.

Key words: Cardiopulmonary resuscitation, Ventricular fibrillation, Mitochondria, Energy metabolism

Yanling Luo, Longyuan Jiang, Yue Fu. Protective effect of cardiopulmonary resuscitation on brain mitochondrial function in a rat model of ventricular fibrillation[J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2015, 01(06): 25-27.

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Figures/Tables 4

References 9

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项目	MAP(mmHg)	HR(次/min)	M_ass/g
对照组	141.06±13.41	297.89±18.61	410.06±34.33
缺血组	145.11±14.29	303.14±21.34	404.03±30.10
CPR组	146.16±15.89	301.08±18.87	399.89±31.56

项目	MAP(mmHg)	HR(次/min)	M_ass/g
对照组	141.06±13.41	297.89±18.61	410.06±34.33
缺血组	145.11±14.29	303.14±21.34	404.03±30.10
CPR组	146.16±15.89	301.08±18.87	399.89±31.56

组别	R3	R4	RCR
对照组	63.9±4.89	13.90±1.21	4.71±0.61
缺血组	28.51±2.89^a	12.49±1.35^b	2.28±0.41^a
CPR组	46.24±3.51^ac	11.88±1.10^a	3.87±0.56^ac

组别	R3	R4	RCR
对照组	63.9±4.89	13.90±1.21	4.71±0.61
缺血组	28.51±2.89^a	12.49±1.35^b	2.28±0.41^a
CPR组	46.24±3.51^ac	11.88±1.10^a	3.87±0.56^ac

组别	PCr(μmoL/g)	ATP(μmoL/g)	Lac(mmoL/g)
对照组	3.75±0.22	2.21±0.13	0.96±0.06
缺血组	0.19±0.05^a	0.31±0.06^a	14.41±1.29^a
CPR组	1.26±0.13^ab	0.86±0.10^ab	9.10±1.09^ab

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