Abstract:
Objective To observe the preventive and therapeutic effect of Proantho Cyanidins on the injury of mouse L929 fibroblasts exposed to UVC in vitro.
Methods In the MTT assay for cell proliferation activity, L929 cells were irradiated with 50 mJ ultraviolet ray, OPC (25, 50, 100, 200 μg/mL) was administrated before and after irradiation, and detected at 24 h after irradiation. The cell cycle and cell apoptosis were assayed by flow cytometry. L929 cells were irradiated with 30 mJ ultraviolet ray. OPC (25, 50, 100 μg/mL) was administrated before irradiation, stained by PI and detected 24 h after irradiation.
Results The cells in the normal control group without UVC irradiation had excellent cell activity. Compared with the normal control group, the cell activity of the irradiation control group was significantly decreased (P<0.05). Compared with the irradiation control group, 25, 50, 100 and 200 μg/mL of procyanidins were given 30 min before irradiation, and 25, 50 and 100 μg/mL of procyanidins were given immediately after irradiation, which can significantly prevent the decreased activity of L929 cells caused by irradiation (P<0.05); Compared with normal control group, the apoptosis percentage significantly increased in the irradiation control group 24 h after UVC irradiation, S phase cell percentage significantly increased in OPC 100 μg/mL (P<0.05). Compared with the irradiation control group, the cell apotosis percentage in 25 and 50 μg/mL OPC group were significantly decreased (P<0.05).
Conclusion Procyanidins can prevent and treat the injury of mouse L929 fibroblasts caused by UV irradiation and its inhibitory effect is related to the dosage of procyanidins.
Key words:
Ultraviole,
Procyanidine,
Cell proliferation activity,
Cell apotosis
Bingxin Xu, Yingying Wu, Bingzhu Xu, Yaya Qin, Shaoyan Si, Yan Cui. Protective effects of Procyanidine on UVC induced damage in L929 Cells[J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2018, 04(04): 229-232.