Abstract:

Objective

Toexplore the expression of lysyl oxidase（LOX）and its significance in the damaged spinal cord tissue sections of rats after acute spinal cord injury.

Methods

70 healthy SD female rats were divided into three groups A，B，C，A for pure damage group，B group of cobalt chloride group，group Cas normal control group，A for pure damage group，B group of cobalt chloride group，group C as normal control group，the rats in the A，B group were sacrificed respectively at four times of 1 d，3 d，7 d，14 d after injure，the injured area of spinal cord tissue was made of paraffin，the expression of LOX was detected by immunohistochemistry in tissue of spinal cord injury，and analyzed the differences between A group and B group analysised the correlation between A and B group of different time periods at the same time.Analyze the differece of groups A，B and C by Statistical analysis methods，correnlation analysis of group A and group B in different time windows.Experimental data using SPSS17.0 statistical software package for processing，all data were expressed as（±s）indicate，group were compared using ANOVA and t test，P＜0.05 was considered statistically significant.

Result

Compared with normal control group，expression of LOX increased in pure damage group at the 1 d，decreased slightly at 3 d，reached the peak at 7 d，then significantly reduced at 14 d.The normal control group（group A）and the simple injure group（group B）expression of LOX were 4.96% and 41.43% （t＝4.602，P＝0.006＜0.01）difference was statistically significant；Cobalt chloride group（group B）with simple injure group（A）LOX expression is basically the same curve，however，the positive expression rate higher than the in group A，was 71.30%（t＝2.3055，P＝0.028＜0.05）difference was statistically significant.1d positive expression of LOX in B group than in A group increased（t＝5.208，P ＝0.002＜0.05），3 d positive expression of LOX in B group than in A group increased（t＝3.142，P＝0.021＜0.05），7 d positive expression of LOX in B group than in A group increased（t＝3.007，P＝0.028＜0.05），14d positive expression of LOX in B group than in A group increased（t＝3.246，P＝0.014＜0.05），at the same time each other compared with statistical significance.

Conclusion

Damaged segment of spinal cord cells appear the expression of LOX and gradually began to rise after SCI，which is closely associated with secondary SCI ischemia hypoxia，and there is a correlation between its expression pattern and injury time.

Key words: Spinal cord injuries, Protein-lysine b-oxidase, Rats,sprague-dawloy