Abstract:
Objective To reveal the possible mechanism of intestinal motility disorder in mice with severe acute pancreatitis (SAP) and the effect of Dahuangfuzi decoction on it.
Methods Twenty seven SPF male C57BL/6 mice were randomly divided into control group, severe acute pancreatitis group (SAP) and rhubarb and aconite decoction treatment group (DHFZT), with 9 mice in each group. SAP group and DHZFT group were intraperitoneally injected with 20% L-arginine solution to replicate SAP mouse model, and the control group was intraperitoneally injected with isotonic saline. In DHFZT group, 0.2 mL rhubarb and aconite decoction was used for enema 12, 24 and 36 hours after modeling, while 0.2 mL isotonic saline was used for enema in control group and SAP group. At 48h after modeling, 3 mice in each group were taken to measure the small intestine propulsion rate, and the rest of the mice were taken blood and materials after anesthesia. Detection of serum amylase, blood calcium, endotoxin and TNF in mice by ELISA- α And IL-6 content. Histopathological changes of pancreas and ileum were observed with HE staining. Western blot was used to detect the expression of nNOS, ChAT, VIP and SP proteins in ileum.
Results Compared with the control group, the expression of nNOS and VIP in the SAP group were increased significantly, and the ChAT and SP were decreased significantly; the propulsion rate of small intestine was decreased significantly, with the lower levels of serum amylase, endotoxin, TNF-α and IL-6, and the blood calcium content also significantly decreased. Compared with the SAP group, the expression of nNOS and VIP in the DHFZT group were decreased significantly, and the ChAT and SP were increased significantly; the propulsion rate of small intestine was increased significantly, with the lower levels of serum amylase, endotoxin, TNF-α and IL-6, and the blood calcium content also significantly increased.
Conclusion The mechanism of intestinal motility disorder in the early course of SAP mice may be related to the ENS remodeling, the increased number of inhibitory neurons, the decreased number of excitatory neurons, and therefore the proportion was imbalanced, leading to intestinal motility inhibition. Dahuangfuzi decoction may reverse the remodeling of ENS neurons and promote the recovery of intestinal motility in SAP mice.
Key words:
Severe acute pancreatitis,
Intestinal motility disorder,
Enteric nervous system,
Dahuangfuzi decoction
Yi Song, Yilong Zhong, Xiaoguang Lu, Libin Zhan, Zhiwei Fan, Tong Lu, Haiming Chen, Qinglin Rui. Mechanism of Dahuang Fuzi decoction in alleviating intestinal motility disorder in mice with severe acute pancreatitis[J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2022, 08(04): 216-221.